前面我给大家详细介绍过

☞GO简介及GO富集结果解读

☞四种GO富集柱形图、气泡图解读

☞GO富集分析四种风格展示结果—柱形图，气泡图

☞KEGG富集分析—柱形图，气泡图，通路图

☞ DAVID GO和KEGG富集分析及结果可视化

也用视频给大家介绍过

☞ GO和KEGG富集分析视频讲解

最近有粉丝反映说，利用clusterProfiler这个包绘制GO富集分析气泡图和柱形图的时候，发现GO条目的名字都重叠在一起了。

气泡图

柱形图

这个图别说美观了，简直不忍直视。经过我的认真研究，发现跟R版本有关。前面我给大家展示的基本都是R 3.6.3做出来的图。很多粉丝可能用的都是最新版本的R 4.1.2。

我们知道R的版本在不停的更新，相应的R包也在不停的更新。我把绘制气泡图和柱形图相关的函数拿出来认真的研究了一下，终于发现的症结所在。

dotplot这个函数，多了个 label_format 参数

我们来看看这个参数究竟是干什么用的，看看参数说明

label_format :

a numeric value sets wrap length, alternatively a custom function to format axis labels. by default wraps names longer that 30 characters

原来这个参数默认值是30，当标签的长度大于30个字符就会被折叠，用多行来展示。既然问题找到了，我们就来调节一下这个参数，把他设置成100，让我们的标签可以一行展示。

是不是还是原来的配方，还是熟悉的味道

同样的柱形图，我们也能让他恢复原来的容貌。

关于如何使用R做GO和KEGG富集分析，可参考下文

GO和KEGG富集分析视频讲解

Term_Name GeneHitsInSelectedSet AllGenesInSelectedSet GeneHitsInBackgroundAllGenesInBackgroundp-value enrichFactorGeneListInSelectedSets Qvalue

00941 Flavonoid biosynthesis14 492 41 38573.30E-042.676878842 "[FvH4_2g26480, FvH4_2g05780, FvH4_4g23870, FvH4_5g35170, FvH4_5g14010, FvH4_7g01160, FvH4_3g44420, FvH4_7g20870, FvH4_4g06180, FvH4_5g01170, FvH4_6g28410, FvH4_3g40570, FvH4_5g22390, FvH4_7g25890]" 0.04909626

00360 Phenylalanine metabolism 14 492 46 38570.001221701 2.38591375 "[FvH4_2g05780, FvH4_4g23870, FvH4_5g35170, FvH4_6g16060, FvH4_4g06180, FvH4_4g25490, FvH4_6g16460, FvH4_6g27650, FvH4_4g09340, FvH4_7g19130, FvH4_3g40570, FvH4_6g26610, FvH4_6g27940, FvH4_6g26600]" 0.091016736

00945 Stilbenoid, diarylheptanoid and gingerol biosynthesis 9 492 31 38570.012547314 2.275963808 "[FvH4_2g05780, FvH4_4g23870, FvH4_5g35170, FvH4_6g28410, FvH4_3g40570, FvH4_5g22390, FvH4_6g26800, FvH4_3g44420, FvH4_4g06180]"0.467387431

00270 Cysteine and methionine metabolism17 492 94 38570.083418875 1.417769417 "[FvH4_4g21340, FvH4_1g10540, FvH4_4g01140, FvH4_2g02530, FvH4_6g27650, FvH4_1g18690, FvH4_5g05120, FvH4_3g14020, FvH4_6g26610, FvH4_4g13980, FvH4_1g18490, FvH4_6g26600, FvH4_1g21920, FvH4_1g26460, FvH4_2g05040, FvH4_2g41260, FvH4_4g13280]"0.654179598

04120 Ubiquitin mediated proteolysis23 492 126 38570.04529262 1.431007227 "[FvH4_7g29370, FvH4_6g11010, FvH4_6g38720, FvH4_5g03910, FvH4_3g09200, FvH4_6g17370, FvH4_3g39370, FvH4_4g01260, FvH4_2g39250, FvH4_5g30320, FvH4_3g00910, FvH4_5g29350, FvH4_6g35920, FvH4_5g33030, FvH4_1g05910, FvH4_5g22570, FvH4_4g14790, FvH4_1g25030, FvH4_4g17530, FvH4_7g16630, FvH4_6g09540, FvH4_6g10930, FvH4_3g18500]"0.674860033

00260 Glycine, serine and threonine metabolism 11 492 49 38570.0408107 1.759872242 "[FvH4_1g08890, FvH4_7g07540, FvH4_5g38450, FvH4_2g05310, FvH4_2g22570, FvH4_1g21920, FvH4_2g16830, FvH4_2g36660, FvH4_1g19090, FvH4_4g13290, FvH4_4g25490]"0.675643816

00670 One carbon pool by folate 5 492 18 38570.069014744 2.177619693 "[FvH4_7g07540, FvH4_5g38450, FvH4_1g00040, FvH4_1g19090, FvH4_4g13290]"0.685546458

03015 mRNA surveillance pathway 20 492 114 38570.082844862 1.375338753 "[FvH4_7g29390, FvH4_6g17300, FvH4_5g13570, FvH4_3g29340, FvH4_4g03530, FvH4_2g38640, FvH4_1g18700, FvH4_1g18000, FvH4_2g34040, FvH4_5g33710, FvH4_6g06810, FvH4_5g25490, FvH4_5g03260, FvH4_2g15670, FvH4_4g07000, FvH4_4g36800, FvH4_5g25550, FvH4_2g06580, FvH4_5g05510, FvH4_6g09230]" 0.685771358

00603 Glycosphingolipid biosynthesis - globo and isoglobo series3 492 9 38570.096237762 2.613143631 "[FvH4_7g21240, FvH4_6g11740, FvH4_3g04760]"0.71697133

00400 Phenylalanine, tyrosine and tryptophan biosynthesis 9 492 37 38570.038722924 1.906888596 "[FvH4_7g11530, FvH4_6g27650, FvH4_6g26610, FvH4_4g21980, FvH4_6g26600, FvH4_2g22570, FvH4_6g47770, FvH4_5g36810, FvH4_1g20450]"0.721214462

00071 Fatty acid degradation8 492 35 38570.068800169 1.791869919 "[FvH4_1g26810, FvH4_1g08890, FvH4_5g05130, FvH4_2g14760, FvH4_4g18500, FvH4_1g25230, FvH4_2g37760, FvH4_6g40560]" 0.732230372

04712 Circadian rhythm - plant 5 492 14 38570.024734738 2.799796748 "[FvH4_2g29440, FvH4_7g29370, FvH4_1g17250, FvH4_7g01160, FvH4_5g22570]"0.737095202

03410 Base excision repair 8 492 34 38570.05939718 1.844571975 "[FvH4_4g29150, FvH4_4g36650, FvH4_2g21980, FvH4_6g11530, FvH4_2g39710, FvH4_4g35010, FvH4_2g40160, FvH4_4g35030]" 0.737514985

00130 Ubiquinone and other terpenoid-quinone biosynthesis 8 492 34 38570.05939718 1.844571975 "[FvH4_4g28800, FvH4_4g09340, FvH4_3g40570, FvH4_6g26610, FvH4_6g27940, FvH4_6g26600, FvH4_4g06180, FvH4_6g16460]" 0.737514985

00460 Cyanoamino acid metabolism7 492 32 38570.103957465 1.714875508 "[FvH4_4g26180, FvH4_7g07540, FvH4_5g38450, FvH4_7g05220, FvH4_1g19090, FvH4_4g13290, FvH4_3g43510]"0.737602967

00310 Lysine degradation8 492 30 38570.030124137 2.090514905 "[FvH4_1g08890, FvH4_5g05130, FvH4_3g23070, FvH4_1g16260, FvH4_1g25230, FvH4_2g36660, FvH4_6g40560, FvH4_3g25420]" 0.748082742

00785 Lipoic acid metabolism2 492 4 38570.081725815 3.919715447 "[FvH4_6g44960, FvH4_4g37350]" 0.761071655

00601 Glycosphingolipid biosynthesis - lacto and neolacto series2 492 4 38570.081725815 3.919715447 "[FvH4_6g11740, FvH4_3g04760]" 0.761071655

00940 Phenylpropanoid biosynthesis 26 492 149 38570.056260767 1.367954384 "[FvH4_2g05780, FvH4_4g23870, FvH4_5g35170, FvH4_7g32980, FvH4_2g30540, FvH4_2g26620, FvH4_7g05220, FvH4_3g44420, FvH4_6g16060, FvH4_4g06180, FvH4_6g16460, FvH4_3g43510, FvH4_7g19130, FvH4_4g26180, FvH4_6g28410, FvH4_6g27940, FvH4_4g36130, FvH4_3g46010, FvH4_1g16790, FvH4_6g30610, FvH4_4g09340, FvH4_3g15230, FvH4_3g40570, FvH4_5g22390, FvH4_6g27610, FvH4_5g21320]" 0.762077663

00450 Selenocompound metabolism 4 492 15 38570.113762224 2.090514905 "[FvH4_2g38710, FvH4_7g04540, FvH4_6g24170, FvH4_2g41260]" 0.770480519

00563 Glycosylphosphatidylinositol(GPI)-anchor biosynthesis 3 492 13 38570.224775136 1.809099437 "[FvH4_5g04770, FvH4_2g15820, FvH4_1g19740]"0.797416555

03008 Ribosome biogenesis in eukaryotes 6 492 33 38570.238324783 1.425351072 "[FvH4_1g27070, FvH4_1g17250, FvH4_1g16590, FvH4_2g38700, FvH4_3g27590, FvH4_1g22910]" 0.807054378

00860 Porphyrin and chlorophyll metabolism 8 492 47 38570.244407142 1.334371216 "[FvH4_3g20600, FvH4_5g33760, FvH4_7g25640, FvH4_2g27000, FvH4_3g20590, FvH4_1g04700, FvH4_2g23050, FvH4_4g37020]" 0.809259204

00053 Ascorbate and aldarate metabolism 8 492 47 38570.244407142 1.334371216 "[FvH4_1g08890, FvH4_5g05130, FvH4_3g33910, FvH4_6g20720, FvH4_7g08190, FvH4_7g13380, FvH4_1g25230, FvH4_5g20650]" 0.809259204

00944 Flavone and flavonol biosynthesis 2 492 5 38570.124932679 3.135772358 "[FvH4_6g17070, FvH4_5g14010]" 0.809346486

00040 Pentose and glucuronate interconversions 15 492 96 38570.236599083 1.224911077 "[FvH4_2g26010, FvH4_6g41430, FvH4_6g17310, FvH4_6g17430, FvH4_3g01680, FvH4_5g27090, FvH4_6g53340, FvH4_2g19540, FvH4_5g33570, FvH4_1g00260, FvH4_2g25970, FvH4_7g08190, FvH4_1g26360, FvH4_4g21500, FvH4_1g27720]"0.819843336

03450 Non-homologous end-joining2 492 7 38570.221439565 2.239837398 "[FvH4_4g35010, FvH4_4g35030]" 0.824862379

00942 Anthocyanin biosynthesis 2 492 7 38570.221439565 2.239837398 "[FvH4_3g19220, FvH4_7g33840]" 0.824862379

这是参考以下教程用自己的数据实现一遍，

R语言ggplot2画图系列——Pathway富集分析气泡图 - 生信技能树 - Powered by Discuz! http://www.biotrainee.com/forum.php?mod=viewthread&action=printable&tid=927

R语言ggplot2绘图教程——Pathway富集分析气泡图 - CSDN博客 http://blog.csdn.net/sinat_38163598/article/details/72827851

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